FastQCFastQC Report
Sun 14 Apr 2019
SRR6576729_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576729_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297793
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5910.19846000409680548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.1349930992333601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3850.12928443583294436No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3700.12424737989140107No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3280.11014362325507986No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3040.10208433374861062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.040.3908771
TACATGG18800.035.24492
GTAATAC801.1569187E-535.2448963
GTACATG19250.034.7000731
ACATGGG19450.033.8254363
ATGGGAG5250.032.2239075
AACGCAG11800.031.8597936
ATCAACG12000.031.720413
TCAACGC11950.031.4598834
CAACGCA12000.031.32885
CATGGGG6100.030.8152124
CATGGGA11550.029.7013284
TAACACC953.7467453E-529.6799144
CCTTATA804.7765224E-429.3707492
GTCTAGC650.006121065528.9480571
TATACAG1802.3646862E-1028.7180655
ACGCAGA13200.027.768717
CGCAGAG13450.027.2754638
TATCAAC14600.027.0371842
GCCTAAT700.00878860726.8803391
Produced by FastQC (version 0.11.2)