FastQCFastQC Report
Sun 14 Apr 2019
SRR6576729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297793
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6350.21323536819199915No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4210.14137337009264825No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3820.1282770246446357No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3700.12424737989140107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.11383746427887828No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3140.1054423710429728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAT558.8055094E-751.258621
ATAGACT1305.456968E-1239.758294
GTATCAA9900.039.39321
TGGACCG1003.320747E-837.5896575
TACATGG19700.035.5384452
GTACATG20100.035.2987441
ACATGGG19800.035.1216473
AACGCAG11600.032.8099376
ATCAACG11700.032.529513
CATGGGG5950.031.5879464
GTAATAG1052.0630032E-631.3247131
CAACGCA12250.031.0695
TCAACGC12350.030.817434
CATGGGA12050.029.6354
CTATAGC650.006155215728.9151211
AATAGAC1154.1916464E-628.6008263
ATATTAC1154.1916464E-628.6008263
CGCAGAG13800.027.5793698
TATCAAC13850.027.4798032
ACGCAGA13850.027.4798037
Produced by FastQC (version 0.11.2)