FastQCFastQC Report
Sun 14 Apr 2019
SRR6576728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322553
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6410.1987270309065487No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4250.1317612919427195No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3790.11750006975597808No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3680.11408977749393122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.11191959150899232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA255.554906E-575.1956563
CCGCTAA352.9351743E-453.7111854
CGCTAAA352.9351743E-453.7111855
GTATCAA9200.048.033861
TACATGG21600.037.0000762
GTACATG21650.036.6974831
ATCAACG12050.036.2717673
ACATGGG21900.036.267313
CATGGGT4000.035.2479674
TCAACGC12650.034.9228864
CAACGCA12700.034.7853935
AACGCAG12750.034.2803736
CATGGGA11750.032.7981074
GGGTACC2850.031.3315247
ACGCAGA14650.029.8344557
TGGGTAC3000.029.7649486
GGTACCT2850.029.6824958
CGCAGAG14900.029.6492948
ATGGGAT4000.029.3733045
TATCAAC15100.029.2656632
Produced by FastQC (version 0.11.2)