FastQCFastQC Report
Sun 14 Apr 2019
SRR6576727_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576727_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334082
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6560.1963589777360049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.14816721643189396No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4020.1203297394052957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.11554049604588096No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3590.10745864787686855No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3460.10356738764734406No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3360.10057411054770984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9600.051.5035321
TACATGG21150.041.769132
GTACATG21250.041.6598661
ACATGGG21850.040.0008743
AACGCAG12350.039.951246
ATCAACG12450.039.630353
TCAACGC12700.038.8502244
CAACGCA12850.038.396725
ATGGGTA3550.037.0627485
CATGGGT5250.035.802114
TATCAAC14400.034.590062
CATGGGA12200.034.279794
GTATAAC550.002689210734.2465061
ACGCAGA14500.033.7084127
CGCAGAG14750.032.838128
GGGTACC3300.031.3315377
CATGGGG6500.031.0858734
ATGGGAG6050.031.067955
GGTACCT3250.030.3856858
TGGGTAC3450.029.964816
Produced by FastQC (version 0.11.2)