FastQCFastQC Report
Sun 14 Apr 2019
SRR6576726_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576726_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363172
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6790.1869637527122135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.1445596026125362No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5200.14318284449241683No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4050.1115174077296708No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3930.10821318824138426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.045.2137871
GTACATG23500.039.0810661
ACATGGG23100.039.065283
TACATGG23700.038.27462
GTACTAG757.324421E-637.6781541
ACTGCGC500.001701571737.5951548
CATGGGG8900.034.3261454
CATGGGA12100.034.1821174
ATCAACG11650.033.485273
CAACGCA11800.033.0596165
TCAACGC11850.032.9201244
AACGCAG12100.032.6283876
ATGGGAT4300.028.4188545
TATCAAC13900.028.4031282
ATGGGAG5500.028.200255
GGTATCA4700.028.05821
TATATAC1555.062975E-827.2905623
ACGCAGA14500.027.2240777
ATGGGGG4550.026.857385
CCGGTCA700.00883376126.853689
Produced by FastQC (version 0.11.2)