FastQCFastQC Report
Sun 14 Apr 2019
SRR6576726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363172
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8540.23515028691639223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.138226515259987No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4510.12418358243476921No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4230.1164737369621006No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4180.1150969788419812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8900.051.2239721
GTACATG23400.038.965241
ATCAACG12000.037.599453
TACATGG24200.037.288712
ACATGGG24000.037.207793
TCAACGC12150.037.1352584
AACGCAG12850.036.9410936
CAACGCA12650.036.0389985
CATGGGA12350.032.3477024
CATGGGT6400.032.3120274
TATCAAC14500.032.0891842
ACGCAGA14650.032.081447
CGCAGAG14900.031.8585938
AGCGTAA300.004160281331.33287236-37
CATGGGG7900.030.3413264
ATGGGGG3250.028.9226515
GGTACCT3850.028.077518
GTACTAA856.8076316E-427.6466541
TATTAGG1057.408578E-526.856752
AGTGCGT700.00882884126.8567498
Produced by FastQC (version 0.11.2)