FastQCFastQC Report
Sun 14 Apr 2019
SRR6576725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394230
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13340.33838114806077674No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11100.28156152499809756No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9500.24097607995332673No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7900.20039063490855594No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC6320.16031250792684473No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG5710.14483930700352587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.13748319508916115No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5050.1280978109225579No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4900.12429292544961063No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4340.11008801968394086No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4330.10983436065241103No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG4320.10958070162088121No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3960.10044897648580778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATA352.938486E-453.7018938
GTATCAA9100.049.6844141
TATAAGC405.675059E-446.9951172
GGTATCA3450.045.0489961
TAGTACT450.001014295441.768144
AACGCAG11650.037.5106546
CAACGCA11700.037.3503575
TCAACGC11700.037.3503574
ATCAACG11900.036.722623
GTACATG19600.035.56281
TACATGG20100.034.603372
ACATGGG20300.034.4895743
CGGATAA550.002717926634.173939
TATCAAC13350.033.4422152
CATGGGG12000.032.8924064
CTTAGGT3050.032.3531883
GTCTTAG3900.031.3977871
ACGCAGA14400.030.6734777
TTAGGTA3100.030.3155824
GTGCAAG1751.6734703E-1029.6036281
Produced by FastQC (version 0.11.2)