FastQCFastQC Report
Sun 14 Apr 2019
SRR6576724_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576724_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119812
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.29880145561379495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1680.14021967749474176No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1530.12770006343271126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.12185757687043035No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1270.10599939905852503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACG301.3672869E-462.656376
ATGGGAC1100.046.992285
GTATCAA6400.044.110491
CATGGGT2400.043.0762524
GGGTTAG450.001011135941.7709167
TACATGG12350.041.0944632
ATGGGTA704.605712E-640.2790955
GTACATG12900.040.121171
ACATGGG12900.038.9780923
ACAACTA250.001700712137.59382216-17
AACGCAG7650.036.856696
CATGGGA7550.036.1000254
ATGGGAG3050.035.4368025
ATCAACG8250.035.3154073
CAACGCA8250.034.7458045
TCAACGC8250.034.7458044
TATCAAC8500.034.2767222
GATACCG550.002709531534.17627
TGGGTTA702.1754854E-433.565916
CGCAGAG8900.031.7066568
Produced by FastQC (version 0.11.2)