FastQCFastQC Report
Sun 14 Apr 2019
SRR6576722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339310
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6180.18213433143732868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.13645339070466536No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3890.11464442545165189No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3540.10432937431846982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.038.782571
ACCGTCG550.002706606234.2016648
GTACATG22100.034.072031
TACATGG22700.032.7085272
ACATGGG23050.031.600253
CATGGGT4550.030.9841524
AACGCAG13800.030.3068436
ATCAACG13900.030.0888083
TCAACGC14000.029.5382254
CAACGCA14050.029.4331075
TATCAAC14800.028.57662
TACGACG1005.3183132E-528.195585
ACTAATA1057.4143376E-526.8529322
CATGGGA12950.026.852934
ATGGGAT3400.026.2605885
CGCAGAG16400.025.5209068
ACGCAGA16400.025.50217
CGACGGT950.001303882324.7329647
CTATAGA1151.3621693E-424.5540831
CTACGAC1151.3758794E-424.5178954
Produced by FastQC (version 0.11.2)