FastQCFastQC Report
Sun 14 Apr 2019
SRR6576721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6440.17171043860818558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.044.80181
GTACATG23950.039.4591751
TACATGG24050.039.29512
ACATGGG24350.038.9884643
ATGGGTA3600.037.8598755
CATGGGT5900.036.642874
ATCAACG11350.036.4393423
CAACGCA11650.035.500995
TCAACGC11750.035.1988534
AACGCAG12050.034.712566
TATCAAC13200.031.701042
GATCTAT902.582808E-531.3448471
CGCAGAG13750.030.7626348
CATGGGG10450.030.5827334
ACGCAGA14050.030.105787
GGGTACC3350.029.4617257
CATGGGA11950.027.9237754
ATACCGT856.808766E-427.6461586
GTCCTAC1205.812797E-627.426741
ATGGGAC2900.025.930195
Produced by FastQC (version 0.11.2)