FastQCFastQC Report
Sun 14 Apr 2019
SRR6576719_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576719_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352969
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10050.2847275539778281No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7840.2221158232026042No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6820.193218101306347No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6410.18160235034804756No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5620.1592207814283974No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5420.15355456144873914No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5100.1444886094812859No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5000.14165549949145675No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4760.13485603551586683No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4400.12465683955248194No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4370.12380690655553321No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4350.12324028455756737No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4090.11587419858401161No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3930.11134122260028502No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3920.1110579116013021No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3910.11077460060231918No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3770.1068082466165584No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3720.10539169162164383No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3710.10510838062266091No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3700.10482506962367799No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG3620.10255858163181468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATGT352.9362034E-453.708786
GTATCAA9950.042.1373671
GTACATG22800.039.2571951
ACATGGG23100.039.060933
TACATGG23350.038.6427232
ATAGACG500.001701286337.5961468
ATCAACG11450.035.297693
CATGGGT6950.035.1618654
CAACGCA11850.034.5027925
ACTTGTA1107.6775905E-834.1783148
AACGCAG11850.034.106216
TCAACGC11900.033.9629064
AGACCCG1001.4086236E-632.896635
CATGGGC4400.029.9060254
ATGGGTA4300.029.5086025
ATGGGGA5100.029.4871735
ATGGGAT2151.8189894E-1228.4156915
ACGCAGA14100.028.3304317
CGCAGAG14150.028.2303228
CATGGGG9450.027.3516924
Produced by FastQC (version 0.11.2)