FastQCFastQC Report
Sun 14 Apr 2019
SRR6576718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382590
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13850.3620063253090776No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7820.204396351185342No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7260.18975927232807965No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7060.1845317441647717No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6950.18165660367495232No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5800.1515983167359314No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5500.14375702449096944No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5440.14218876604197705No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5440.14218876604197705No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5060.1322564625316919No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4920.1285971928173763No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4620.12075590057241434No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4590.11997177134791814No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4520.11814213649076034No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4360.11396011396011395No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4280.11186910269479076No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG4110.10742570375597899No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4110.10742570375597899No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4090.10690295093964818No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3900.10193679918450561No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG3860.10089129355184401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.10010716432734781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA250.00523942956.3835333
ATAGTAC352.9392648E-453.6986053
GTATCAA9550.040.3811841
TTAAGAC608.8282206E-539.1552283
GGTAATC651.4141545E-436.1432888
GTACATG21100.035.8849221
TACATGG21550.035.5720522
ACATGGG21300.035.5154463
CTAAATC550.002718643534.1718373
GGGTACC4550.034.0779577
ATGGGTA4750.033.6322825
TGGGTAC4650.033.34516
GAATAAG851.7442773E-533.1971551
CGCCGGT1001.4111192E-632.8903927
GGTACCT4800.032.3030628
CATGGGT7100.031.765374
AACGCAG11950.031.455256
ATCAACG12050.030.8042813
TCAACGC12150.030.5507474
ATAACGC1102.9683742E-629.9003563
Produced by FastQC (version 0.11.2)