FastQCFastQC Report
Sun 14 Apr 2019
SRR6576717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419836
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14160.3372745548261702No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8290.19745805505006717No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8090.19269429015139244No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7400.17625930125096465No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6800.1619680065549405No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6160.14672395887918138No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6100.14529482940957897No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5510.13124172295848857No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5430.12933621699901865No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5380.12814527577434998No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5340.12719252279461504No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5130.1221905696510066No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5090.12123781667127163No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4820.11480673405806076No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4790.11409216932325955No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4640.11051934564925352No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4330.10313551005630771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10750.048.5465971
AACGCAG13450.037.7390146
ATAACTC500.001700966537.599244
GGTACCT5050.037.226978
TCAACGC13850.036.649084
GTACATG23750.036.424911
TACATGG24050.035.9705472
CAACGCA14350.035.6996235
ATCAACG14150.035.5399173
TGGGTAC5450.035.3570826
CAAACGC550.002710568334.1933442
ACATGGG25300.033.623823
GGGTACC5600.033.5707477
ATGGGTA5850.032.13615
ACGCAGA16350.031.045247
CGCAGAG16650.030.4858678
CATGGGT8150.029.9871224
TATCAAC17800.029.0547372
ACTTATT650.00613965728.9328292
TGCGACT650.006150387728.92249194
Produced by FastQC (version 0.11.2)