FastQCFastQC Report
Sun 14 Apr 2019
SRR6576716_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576716_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362404
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10550.2911115771349102No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7170.19784549839405746No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6590.18184126003024248No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5980.1650092162338164No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5800.16004238363814968No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5570.15369587532146445No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5470.15093652387942738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.14872904272579773No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5130.14155472897650137No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5070.1398991181112791No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4780.13189699892937162No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4700.12968951777574197No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4650.12830984205472346No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4210.11616869570976038No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4120.11368527941192702No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC4100.11313340912351962No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3910.10789064138364919No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3740.10319974393218619No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3680.10154413306696394No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3630.10016445734594541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10550.049.092371
GTACATG23750.041.0374641
TACATGG24150.040.0907482
ACATGGG24100.039.1934133
AACGCAG13200.038.0928966
ATCAACG13250.036.885153
ATGGGAT2950.036.638695
CATGGGT6550.036.590054
CAACGCA13650.035.8042685
TCAACGC13750.035.5438734
ATGGGTA4200.034.6853875
CATGGGG9600.034.2658044
AGTACTA550.002716519434.1768045
CATGGGA9600.033.286784
GGCTTAT851.7301918E-533.23581
ACGCAGA15500.031.8340387
CGCAGAG15850.031.7415688
GGTACCT3500.030.8982358
ATGGGGG4450.030.6247165
TAAGGTG804.778604E-429.370695
Produced by FastQC (version 0.11.2)