FastQCFastQC Report
Sun 14 Apr 2019
SRR6576713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200767
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.245060194155414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.11655301917147737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6500.047.828141
GCACCGT405.666725E-446.9978836
ATCAACG7150.042.725353
TATCAAC7600.041.4426652
TCAACGC7450.041.0048644
AACGCAG7450.040.374026
CAACGCA7600.040.1955575
GTACATG9500.035.6994741
ACGCAGA8500.035.3866427
CGCAGAG8800.034.180288
TACATGG10350.032.7023242
ATGGGAT1755.456968E-1232.227125
ACATGGG10250.032.096113
CATGGGA6050.031.8498024
GGTATCA3250.030.4360891
GTACTTG953.6813355E-529.749561
GCAGAGT10150.029.6341329
CTTTAGC650.00613611828.9289782
GTAACAT650.00614359328.9217743
GACAGTA700.00882091426.8559327
Produced by FastQC (version 0.11.2)