FastQCFastQC Report
Sun 14 Apr 2019
SRR6576713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200767
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.19823975055661539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT407.986515E-658.7789731
CATGCGT250.00522987556.39970494
ATAACGC250.00522987556.3997043
CGCTAAA352.9317394E-453.7139975
CCCGCTA352.9317394E-453.7139973
CCGCTAA405.665613E-446.9997524
ATGGGTG1209.640644E-1139.166465
GTATCAA5050.039.108381
TGCCCGC651.4052563E-436.1716731
TTAGTAC550.002711765534.1816373
CATGGGT2350.033.999824
ATCAACG5850.032.9399953
GTACATG10050.031.3487831
GGGTACC1052.0542484E-631.3331687
TAATACC600.00415298931.3331664
TACATGG9750.031.3331662
TCAACGC6300.030.587144
CAACGCA6350.030.3462965
ACATGGG10200.029.9508233
TATCAAC6550.029.4196932
Produced by FastQC (version 0.11.2)