FastQCFastQC Report
Sun 14 Apr 2019
SRR6576710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395211
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12470.31552765484766365No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7460.18875992824086374No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6350.16067366545971645No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6330.16016760667086696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.15940851848759272No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5870.14852825452732843No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5480.13866010814476318No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5230.13233437328414444No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5210.13182831449529492No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4680.11841775659078314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4560.11538140385768615No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4420.11183899233573964No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4310.10905566899706738No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4170.1055132574751209No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4110.10399508110857238No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4060.10272993413644864No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3980.10070569898105063No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3960.10019964019220114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.054.9230961
ATCAACG13550.044.3983463
CAACGCA13600.043.889535
TCAACGC13750.043.752554
AACGCAG13900.042.936846
GGTACCT4750.039.5988358
ATGGGTA4600.037.80425
TATCAAC16800.036.3735052
ACGCAGA16300.036.3265467
GGGTACC5050.036.2922947
CGCAGAG16550.035.8004578
CATGGGT6850.034.992564
ACATGGG22350.034.697853
GTACATG23950.033.240511
TACATGG23950.033.168992
GCAGAGT17800.033.02229
TGGGTAC5700.030.5047876
ATCTTAG953.6883488E-529.7518621
TATAGGT804.775383E-429.3748824
CATGGGG10850.029.0229264
Produced by FastQC (version 0.11.2)