FastQCFastQC Report
Sun 14 Apr 2019
SRR6576710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395211
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13690.3463972409674832No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7160.18116904640812123No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7060.17863875246387373No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6550.16573425334821146No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5990.15156460726042545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.13511769662281667No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5290.13385254965069293No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4990.12626166781795042No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4960.12550257963467618No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4920.12449046205697716No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4810.1217071387183049No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4790.1212010799294554No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4580.11588746264653564No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4550.1151283744632614No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4540.11487534506883663No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4510.11411625688556239No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4340.10981475718034163No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4260.10779052202494364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAG250.005237326556.3895953
GCAAACG602.5387635E-854.8648721
GTATCAA8600.049.214341
ATCAACG10400.039.7618983
CAACGCA10450.039.5716485
TCAACGC10450.039.1219674
GTACATG21800.037.7510641
ACATGGG21250.037.5930673
AACGCAG11050.036.99776
TACATGG22350.036.405852
CATGGGT7000.035.579154
ATGGGTA4300.033.8774725
CATGGGG11550.031.3275554
GTAATAC600.004161463631.3275533
ACGCAGA13150.031.0893217
ACGCAAA1401.8877472E-830.2087145
CGCAGAG13750.029.7326988
TATCAAC14400.029.3956182
AACGCAA1452.6555426E-829.1670324
TATCGCT650.006154890628.9177444
Produced by FastQC (version 0.11.2)