FastQCFastQC Report
Sun 14 Apr 2019
SRR6576702_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576702_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216900
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.18902720147533425No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4020.18533886583679116No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2550.11756569847856155No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2330.10742277547256801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5550.053.4809461
GTATATA902.4374458E-1047.1141661
TCAACGC6200.044.7207644
CAACGCA6250.044.3629955
AACGCAG6450.043.7166
ATCAACG6550.043.0485763
CGCAGAG7350.039.0024038
ACGCAGA7400.038.103817
GTACATG10750.036.3765221
TATCAAC7950.036.0588262
TACATGG10950.035.621552
GGTATCA2150.035.0617071
GTTCTAG550.002680088434.2648471
TAAGGCC702.1806308E-433.5676424
ACATGGG11550.033.36423
GTCTAGA851.7194528E-533.2570571
GCAGAGT8750.032.762029
CATGGGG5150.031.9381434
GTATTAC600.004104560731.4094451
GCTGAAT753.2690176E-431.3297989
Produced by FastQC (version 0.11.2)