FastQCFastQC Report
Sun 14 Apr 2019
SRR6576701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257488
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6090.23651587646802957No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3590.13942397315603056No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2900.11262660784191883No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2820.10951966693593489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.1071894612564469No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2610.10136394705772696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.041.0982361
GTACATG16900.038.6682321
GTAACTC500.001701974537.5892033
TACATGG17100.037.3911672
ACATGGG17800.035.635833
CACGATC550.002716935734.1728
ATCAACG9250.032.509583
TCAACGC9500.031.6540644
CATGGGT5350.031.6170854
AACGCAG9700.031.4858026
CAACGCA9900.030.8497265
TATATTG650.006154050628.9147725
CATGGGA9300.028.7981784
ATGGGTG2500.028.19195
TATCAAC10850.028.1650052
ACGCAGA10850.028.1485967
CGCAGAG10750.027.9733588
GTATATA1853.237801E-1027.9542081
CCCTAGG700.00881080426.865082
GTCTATA700.00881080426.865081
Produced by FastQC (version 0.11.2)