FastQCFastQC Report
Sun 14 Apr 2019
SRR6576700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286817
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6580.22941457444991056No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3370.11749652217267458No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3170.11052343480337638No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3010.10494496490793782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.10355034743407818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT156.892922E-494.0135961
GTATCAA7100.044.3585321
TACATGG17500.037.068222
GTACATG17550.036.9626121
ACATGGG17900.035.9709973
TCAACGC8850.035.049814
ATCAACG8850.034.518753
GGTATGA550.002711701234.1867641
AACGCAG9100.034.08696
CAACGCA9300.033.3538485
TATTAGG902.5846371E-531.3378662
GCCTATC600.00415288231.3378661
CATGGGT4550.030.9880894
CGCAGAG10100.030.711968
CATGGGG6650.029.683334
ACGCAGA10500.029.5419797
TATCAAC11000.028.204082
CATGGGA8700.027.5509054
ATGGGAT2550.025.8031565
GCAGAGT12600.024.6183179
Produced by FastQC (version 0.11.2)