FastQCFastQC Report
Sun 14 Apr 2019
SRR6576697_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576697_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145151
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3520.24250607987544004No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2400.1653450544605273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.14743267356063686No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1970.13572073220301617No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1670.11505260039545026No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1630.1122968494877748No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1620.11160791176085595No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1530.10540747221858615No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480.10196278358399186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC250.00520745256.4529881
GTATCAA5400.040.074651
GTACATG10050.037.9161831
ACATGGG10100.037.224183
TACATGG10300.036.5013732
TGGGTAC1502.7284841E-1134.4633836
GTGTAGG550.00269677534.213931
CATGGGT2250.033.4190374
AATAGAG851.745423E-533.173315
ATGGGAG2300.032.6925355
GGTACCT1658.367351E-1131.3519548
CATGGGC1351.3114914E-831.3303474
GGGTACC1658.54925E-1131.3303477
AACGCAG6000.031.3303476
ATACTGC600.00415105631.3303456
CATGGGA6100.030.8167344
TCAACGC6400.030.1065064
ATCAACG6450.029.8731213
ATGGGTA1751.6916601E-1029.540045
ATGGGAC804.7632167E-429.37225
Produced by FastQC (version 0.11.2)