FastQCFastQC Report
Sun 14 Apr 2019
SRR6576696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160188
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3520.21974180338102728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.1554423552326017No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2150.13421729467875246No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1990.12422903088870577No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1980.12360476440182785No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1980.12360476440182785No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1670.10425250330861238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT408.008359E-658.7427839
TACCGTC558.77053E-751.2664267
ACCGTCG502.9999263E-546.9942258
ATACCGT704.6042605E-640.2933436
GTATCAA5600.038.711171
AACGCAG5550.038.1153266
CCTATAA500.001675352137.7013171
GGTTGCA500.001698682237.5953837
GTACATG9000.037.1776851
ATGGGAG2300.036.7895745
CAACGCA5800.036.4724245
ATAGACT651.4064967E-436.160694
TCAACGC5850.036.160694
ACATGGG9300.035.8885153
ATCAACG6000.035.2566763
TACATGG9650.035.0739972
CATGGGG3400.034.565374
TAGACTT1001.3990648E-632.906235
CGCCGGT600.004152892631.3294837
ACGCAGA6800.031.099127
Produced by FastQC (version 0.11.2)