FastQCFastQC Report
Sun 14 Apr 2019
SRR6576695_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576695_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412974
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11020.2668448861187387No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6520.1578791885203427No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5820.14092896889392553No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5530.13390673504869557No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5370.13003239913408593No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5220.1264002092141394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.11792509940093082No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4830.1169565154222784No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4710.11405076348632118No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4680.11332432550233186No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4480.10848140560906983No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4380.1060599456624388No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4190.10145917176383985No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4190.10145917176383985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.050.5863151
ATCAACG13600.035.9362683
GTACATG23450.035.1293831
TCAACGC14200.034.7487754
AACGCAG14250.034.2970736
ACATGGG22900.034.0652163
CAACGCA14550.033.5899165
TACATGG24350.033.3876382
ATGGGTA3150.032.820925
ACGCAGA16050.030.450677
ATGGGCG1102.9655312E-629.9050065
GGGTAAC650.006102963428.9682331
CTACGGA650.006135585328.9366469
CGCAGAG17450.028.294078
GCTATAA1005.2591608E-528.2440281
TATCAAC17650.027.9565222
ATAACTA700.00883546526.8534764
TAGTACA700.00883546526.8534764
CATGGGT6050.026.4096184
TATAGAT909.514898E-426.1075462
Produced by FastQC (version 0.11.2)