FastQCFastQC Report
Sun 14 Apr 2019
SRR6576690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147189
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3830.26020966240683746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.18751401259605No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2610.17732303365061247No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2220.15082648839247498No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2180.14810889400702498No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2040.13859731365794997No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1990.1352003206761375No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1930.13112392909796247No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1920.1304445305016No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1860.126368138923425No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1830.12432994313433748No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1820.123650544537975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1730.1175359571707125No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1720.11685655857434998No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1710.11617715997798748No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1670.11345956559253749No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1550.10530678243618749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.10394798524346249No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC1490.1012303908580125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGATC608.796164E-539.1594437
ACCCTAG757.2886833E-637.6826971
TGTAGGA651.409037E-436.1471822
ATAGGTC1001.4021316E-632.8939363
TACATGG13900.031.7783152
GTACATG14100.031.7363151
ACATGGG13750.031.4414753
TAGGTCA1102.9495604E-629.903584
GTATTTT804.69998E-429.4396081
CATGGGG4850.029.0668054
GGGAGAC650.00614251228.9177467
GATAGGT1154.1697804E-628.603422
GTATCAA8050.027.5013481
ATGGGGG1904.4019544E-1027.205515
CATGGGT2950.027.0797524
CTGTGCA700.00879017826.8704519
GGGATCC700.00879017826.8704518
AGCGTCA350.00883347726.85219290-91
CGGAAAA350.00883347726.85219274-75
TTTGTCG350.00883347726.85219260-61
Produced by FastQC (version 0.11.2)