FastQCFastQC Report
Sun 14 Apr 2019
SRR6576689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162640
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4740.2914412198721102No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2880.17707820954254797No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2710.16662567634038367No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2510.1543285784554845No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2350.14449090014756516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.1438760452533202No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC2180.1340383669454009No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2180.1340383669454009No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2080.12788981800295132No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2070.12727496310870634No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1990.1223561239547467No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1950.11989670437776685No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1870.1149778652238072No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1860.11436301032956221No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1860.11436301032956221No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1790.11005902606984752No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1780.10944417117560257No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1680.10329562223315297No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1670.10268076733890802No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1650.1014510575504181No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC1630.10022134776192819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC408.00339E-658.7496153
GTACTTG652.7643928E-643.3976751
ATGGGAG2350.039.9997375
GTACATG13150.039.6849481
TACATGG13700.038.091762
ACATGGG14100.037.3330883
GTATCAA6950.036.528981
TGGGTAC1601.8189894E-1235.249776
TGGGAGT959.384985E-734.631356
GGGTACC1502.7284841E-1134.4664427
ACTTAAG550.002706186434.192111
GTCTATC550.002710272634.1815957
AACGCAG7300.034.1230626
CATGGGA7650.033.790634
ACTTGGG851.7456538E-533.1762543
CATGGGG5300.031.924324
GTCTTAA753.257525E-431.3427681
GGGAGTT753.2634096E-431.333137
ACTATAT600.00415071231.3331283
TCTATAT600.00415071231.3331283
Produced by FastQC (version 0.11.2)