FastQCFastQC Report
Sun 14 Apr 2019
SRR6576688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266191
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6770.25432865874503646No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4030.15139505092208227No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3930.14763834990664598No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3530.13261154584490084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.11908742218933022No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3090.1160820613769812No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2990.11232536036154492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT804.627509E-946.9860084
GTACATG19500.040.728851
TACATGG19650.040.417952
ACATGGG19650.040.1712383
CATGGGT5300.039.0072484
GTATCAA7650.037.4730031
CATGGGG5850.034.536724
ATGGGTA3450.032.6859175
CATGGGA9950.032.583264
GCCTAAG600.004157526431.329891
GGTTAGC600.004161352331.3240038
CTAATAG753.274549E-431.3240033
ATGGGAC2450.030.6847385
AACGCAG9550.030.0120056
GGGTACC2350.029.9910667
GCAAACG650.00614909128.9198951
CTAGTAC650.006154739828.9144633
ATCAACG10300.027.8266643
TACAGCG856.814089E-427.6388287
CAACGCA10550.027.6126295
Produced by FastQC (version 0.11.2)