FastQCFastQC Report
Sun 14 Apr 2019
SRR6576687_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576687_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293534
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6170.21019711515531422No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4040.13763311916166443No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3990.13592973897402003No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3710.1263908099232116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.1263908099232116No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2970.10118078314607507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG156.8937195E-494.011425
ACGTGCG301.3697997E-462.66368
GTATCAA8900.043.4492871
CTTACTA500.001700372137.598169
GTACATG19450.034.6717071
ATCAACG10850.034.6644943
AACGCAG11000.034.185976
TACATGG19350.033.772132
TCAACGC11250.033.426284
ACATGGG19600.033.1014983
CAACGCA11450.032.8424155
TACTAAG600.00408809331.438921
TCTATAC753.264054E-431.342483
ATGGGTA3300.031.337145
TCCGAAA300.00416033431.331892-93
CATGGGA10350.030.4288164
GGGTACC2950.030.2697077
TATCAAC13000.029.2931632
GGTCTAA650.00604657829.0205381
ACGCAGA13350.028.515467
Produced by FastQC (version 0.11.2)