FastQCFastQC Report
Sun 14 Apr 2019
SRR6576687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293534
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6950.23676984608256624No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4550.15500759707563688No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3900.13286365463626018No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3340.11378579653464334No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3260.11106038823441237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10935700804676801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.044.351681
ATGGGAG4750.040.5656245
GTACATG20100.038.5925831
TACATGG20450.037.9320762
ACATGGG20750.037.82393
CCAACCG500.001700538937.597419
CATGGGA10900.036.648854
ATCAACG10200.035.0171973
AACGCAG10550.034.3009576
ATGGGAC2350.033.9976585
CAACGCA10750.033.6627965
TCAACGC10800.033.506954
TGCCCGC753.2644358E-431.3418541
TCTATAC902.5886166E-531.3311773
TATCAAC12200.028.90132
CCCTACA1005.3003914E-528.2076662
CATGGGC3350.028.057774
CGCAGAG13000.027.8365428
CATGGGG6650.027.562014
TAGCCCT2053.6379788E-1127.51034
Produced by FastQC (version 0.11.2)