FastQCFastQC Report
Sun 14 Apr 2019
SRR6576686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233896
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6240.2667852378835038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.17272633991175565No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3830.16374799055990696No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3550.15177685809077537No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3470.14835653452816636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3450.1475014536375141No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3200.1368129425043609No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2970.12697951226185997No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2870.1227041078085987No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2830.12099394602729419No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2760.11800116291001128No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2630.11244313712077163No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2490.10645757088620583No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2470.10560248999555359No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2460.10517494955022745No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2420.10346478776892293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGC250.00522071856.427694
TACATGG16900.038.6509132
GTACATG17400.038.153821
ACATGGG17100.037.6492353
TGGGTAC2150.037.1571276
ATGGGTA2300.036.7770045
CATGGGT3850.032.9560174
CATGGGG6800.032.4803434
GGGTACC2350.031.9951217
CATGGGC3050.030.8149784
ATGGGAG2600.030.7260865
GGTACCT2700.027.8654828
CATGGGA7450.027.1233844
GTACACT700.008745884526.9048231
ATGGGGA2450.026.853055
CTAGTAC700.008828456526.8530483
GTATCAA10200.026.3113371
GTACTTT8450.026.1884231
GAACTGT909.503398E-426.1071326
GTACCTG3150.025.3774939
Produced by FastQC (version 0.11.2)