FastQCFastQC Report
Sun 14 Apr 2019
SRR6576685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249438
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6340.25417137725607164No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4160.16677490999767478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.15154066341134872No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3720.149135256055613No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3680.14753165115178923No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3320.13309920701737504No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3240.12989199720972747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.11906766410891684No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2860.11465775062340142No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2790.11185144204170976No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2750.11024783713788595No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2710.10864423223406218No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2690.10784242978215028No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2570.1030316150706789No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2530.1014280101668551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG450.001001600341.8691331
TTAGGCT450.001012544641.77684
ATGGGTA2950.035.0500225
CATGGGG6600.034.893124
GTACATG14700.034.606121
TACATGG14600.034.4443972
ACATGGG14750.033.456843
TAGTGGT600.004155271731.3325984
TAGTACT600.004155271731.3325984
GTATCAA9950.030.297261
GGGTACC2650.030.1502367
CATGGGT4250.029.8581224
CACTCTA2051.8189894E-1229.8041789
TGGGTAC3000.029.7659666
ATGTAGG1005.22861E-528.2616651
GTCCTAG1205.72112E-627.4766161
AACGCAG11300.027.0347656
GTTACAA700.00872948326.915871
GTCTTAC700.00872948326.915871
GGTACCT2800.026.8565148
Produced by FastQC (version 0.11.2)