FastQCFastQC Report
Sun 14 Apr 2019
SRR6576685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249438
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7220.2894506851401952No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4420.17719834187252945No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3940.1579550830266439No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3800.1523424658632606No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3120.1250811824982561No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2960.11866676288296091No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2950.11826586165700494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.11826586165700494No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2800.11225234326766571No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2780.11145054081575381No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2720.10904513346001812No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2700.10824333100810622No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2630.10543702242641459No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2560.10263071384472293No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2500.10022530648898724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC450.001012427541.777783
CTAGGAC651.4103191E-436.1538473
CATGGGT4250.035.3882374
GTATCAA10200.034.5588231
TAGCGCG550.002712950334.1818164
GTAGCGC702.1801192E-433.571433
GTACATG16750.033.110451
TACATGG16650.032.7447472
ACATGGG17200.031.4244173
GACCGTG600.00415479531.3333347
TGCGACG300.00415874531.33333422-23
GTATATA600.00415479531.3333341
TGTAGCG600.00415479531.3333342
TGGGTAC2350.030.06
TTTAAGC953.740636E-529.684213
ATGGGAG2700.029.5925925
AACGCAG11300.029.5309736
GCCTTAT804.7702796E-429.3751
ATCAACG11250.029.2444463
CAACGCA11550.028.4848485
Produced by FastQC (version 0.11.2)