FastQCFastQC Report
Sun 14 Apr 2019
SRR6576684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394142
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8810.22352350168213486No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6390.16212431052767784No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5630.14284191991718723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.13852875359641956No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4940.12533553896818914No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4150.10529200135991597No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4040.10250112903471338No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4030.10224741336878587No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4030.10224741336878587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12250.037.99651
GTACATG23800.033.5827641
TACATGG24050.032.8426862
ACATGGG24900.031.1353323
AACGCAG14500.030.783966
TCAACGC15600.029.5168744
ATCAACG15600.029.2156833
CAACGCA15700.029.0295965
CATGGGG8750.026.8491694
ATGGGTA3700.026.6677555
ACGCAGA17100.026.1033577
CGCAGAG17500.025.506718
TATCAAC18000.025.336332
GGGTACC3750.025.0592237
CATGGGA12800.024.9613364
ATAGCAC950.001305322624.7294963
CATGGGT6300.024.6117384
GGTACCT3850.024.4083358
TACTACT1551.5464575E-624.250864
TGGGTAC4150.023.776076
Produced by FastQC (version 0.11.2)