FastQCFastQC Report
Sun 14 Apr 2019
SRR6576683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438856
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8780.20006562517089888No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5760.1312503417977651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.12487011684926264No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5470.12464225167253039No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5410.123275060612137No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4600.1048179812968263No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4410.1004885429389139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.049.1078951
GTACATG26800.039.0117571
TACATGG27400.038.255122
TATACCG500.00170016237.6032374
ATCAACG15250.036.986793
CAACGCA15650.036.341795
AACGCAG15800.036.2942626
TCAACGC15550.036.2732164
ACATGGG28350.035.6468053
CATGGGT6750.031.3360314
TACGTTA300.00416101931.33245834-35
TATCAAC18550.031.1671032
ATGGGAG6750.030.6396755
CGCAGAG18850.030.1689198
CATGGGA16200.029.8852864
ACGCAGA19300.029.4655027
CTTGCGA650.00615091628.9222749
ATGGGTA4600.028.6111585
CATGGGG9600.027.9086514
GCAGAGT21350.026.636269
Produced by FastQC (version 0.11.2)