FastQCFastQC Report
Sun 14 Apr 2019
SRR6576681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340530
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8270.24285672334302413No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6030.17707690952339No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5460.1603382961853581No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5090.14947288050979357No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4940.14506798226294307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.14418700261357298No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4480.1315596276392682No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4250.12480545032743076No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4070.11951957243121017No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3790.1112970957037559No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3730.1095351364050157No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3540.10395559862567175No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3470.10189997944380817No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3430.10072533991131472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG405.6721084E-446.9982382
GTATCAA10850.042.9778481
ACATGGG25400.041.262233
GTACATG25800.040.1644861
TACATGG26100.040.155582
GTATAAC608.6991684E-539.251651
ATGGGTA3450.035.418965
GGTACCT3350.033.670388
GGGTACC3400.033.1752247
TACACCG851.7516231E-533.1752245
ATGGGAG5250.033.1225665
AACGCAG14400.032.9640436
CATGGGC4150.032.8421444
CATGGGG8450.032.815344
CATGGGA13750.032.8133134
ATCAACG14100.032.332123
CAACGCA14650.032.0807085
TCAACGC14550.031.9781844
GTATGAC600.00411303131.4013211
CCTATCG600.004157671731.3321553
Produced by FastQC (version 0.11.2)