FastQCFastQC Report
Sun 14 Apr 2019
SRR6576680_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576680_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171202
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.2914685576103083No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4040.23597855165243395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2930.17114286048060187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.1495309634233245No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2500.14602633146809033No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2300.1343442249506431No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2130.12441443441081296No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2110.12324622375906823No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1820.10630716930876975No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1800.10513895865702502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.1028025373535356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAG250.005188492656.5093961
TAAGACT558.773259E-751.267154
CTGACTA503.000551E-546.9948929
GTATCAA8450.038.4531361
ATGGGGC906.154605E-736.5515825
GGGTACC1850.035.56377
CGACCCG1107.638482E-834.17815
GGTACCT2000.032.8964238
ATGGGTA2050.032.094075
TACATGG13750.031.7856352
CATGGGT2850.031.3299264
TAGGCAT600.004153398331.3299265
CTAGGCA753.2660834E-431.3299264
TAATGCC600.004153398331.3299263
GTACATG14250.031.0636431
ATCAACG10400.030.7274273
TGGGTAC2300.030.6488426
TCAACGC10450.030.5804084
AACGCAG10500.030.4347886
CATGGGG4350.030.2495844
Produced by FastQC (version 0.11.2)