FastQCFastQC Report
Sun 14 Apr 2019
SRR6576680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171202
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4190.24474013154051938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.17932033504281492No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2390.13960117288349436No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2080.12149390778145115No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2030.11857338115208935No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1810.10572306398289741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT407.9527945E-658.8146931
GCCTAAC250.005205719756.4621051
GGGTACC1600.049.9340827
GGTACCT1800.044.3858538
GATTGGC751.471908E-743.863679
GTATTGA450.001006070341.8237841
CCTAGGT450.001006070341.8237842
ATATGAG853.9315455E-738.7032363
CGGACGG250.0017009337.59742742-43
CATGGGT2650.035.4692734
GTATCAA7950.034.3270651
CCTAAGA550.002695988234.219462
GTACTTG1107.557719E-834.219461
GTACCTG2400.033.289399
TACATGG14150.032.91962
TGGGTAC2150.032.7884566
ACATGGG14000.032.562063
GTACATG14300.032.245261
ATGGGAG2050.032.0953645
ATGGGTA2000.030.547915
Produced by FastQC (version 0.11.2)