FastQCFastQC Report
Sun 14 Apr 2019
SRR6576679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302543
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5260.17385958359638135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.1176692238789197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA250.0052385956.382944
CGCTAAA250.0052385956.382945
GTACATG23300.034.287141
CCGAACT550.00271568534.1771281
TACATGG23250.033.956632
GTACTAG1256.2427716E-933.8353581
ATGGGAG5750.033.5029075
ACATGGG23100.032.9510733
GTATCAA12850.031.8166471
CATGGGT4550.029.9469834
TAAGTAG650.006156190828.914335
AACGCAG14200.028.7870666
ATGGGTA2350.027.9915315
GGGTACC1853.274181E-1027.9374947
GCCTTAC856.8098574E-427.6432671
CTAGTAC856.8164436E-427.6386993
ATCAACG14900.027.1193123
CATGGGA13050.027.0033254
GTATTAA1057.410731E-526.853461
TAATACC700.00883894826.849024
Produced by FastQC (version 0.11.2)