FastQCFastQC Report
Sun 14 Apr 2019
SRR6576678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327690
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5630.17180872165766425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.125423418474778No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3540.10802892978119565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10000.041.9307481
GTACATG22550.035.935571
ACATGGG22950.035.2230263
TACATGG23000.035.1464542
TAGGGCT1107.6703145E-834.180494
CATGGGG7300.034.121964
CATGGGT4350.031.3321154
ATCAACG13550.030.5227973
TCAACGC13450.030.4003034
AACGCAG13450.030.0508756
TACCCTT1102.9608127E-629.9079277
GTATTAC953.6831916E-529.7557071
CAACGCA13900.029.7542345
ATGGGGA4050.029.0112155
CATGGGA13200.028.1276934
GGGTATA856.710545E-427.7136481
TATCAAC15350.027.555932
AGCGTAG350.00883497826.85609836-37
ATGGGAG6150.025.9827295
ACGCAGA15800.025.8787367
Produced by FastQC (version 0.11.2)