FastQCFastQC Report
Sun 14 Apr 2019
SRR6576677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404063
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10050.24872359013322182No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6840.16928053298619275No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6540.16185594820609656No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5910.14626432016789462No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5620.139087221547135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.12225816271225032No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4760.1178034118441926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4560.11285368865746183No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4480.1108737993827695No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4320.1069140208333849No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4280.10592407619603873No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4170.1032017284433368No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4130.10221178380599065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT250.005191161856.5160981
ATTAACG352.9377468E-453.7049523
GTATCAA11350.037.3454441
GGTACCT3750.036.3673558
GTACATG23400.035.8257371
TACATGG23400.035.5495032
ACATGGG23650.034.9706653
CATGGGT6350.034.041334
GGGTACC4200.032.4427267
ATGGGTA4650.030.3173085
ATCAACG14250.029.3492853
AACGCAG14050.029.0981436
AGTGCGT650.006132431328.939548
ACGTGCG650.006132431328.939549
TGGGTAC4750.028.689756
CAACGCA14650.028.547945
TCAACGC14650.028.547944
GTCCTAA856.727004E-427.7039721
ACTACGT700.00883684926.8524766
TACTACG700.00883684926.8524765
Produced by FastQC (version 0.11.2)