FastQCFastQC Report
Sun 14 Apr 2019
SRR6576677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404063
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10660.26382024585275066No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8010.19823641362856784No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6160.15245147415130808No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5910.14626432016789462No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5640.13958219386580806No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5060.12522799662428877No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4670.11557603641016376No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4620.11433860561348107No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4360.10790396547073104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10790396547073104No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4280.10592407619603873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT250.00524147856.3785448
GTACATG25250.039.6520651
TACATGG25050.039.593362
ACATGGG25400.038.8434873
GTACTAA500.001699763737.604311
GTATCAA11800.033.859811
CATGGGA13900.032.4480824
CATGGGT6250.031.5719814
GCAAACG600.00415548431.3369261
ATGGGTA4150.030.5666795
TGGGTAC3700.030.4748886
GGGTACC3600.030.0163567
TAGTGTC1102.9709663E-629.897717
GGTACCT3650.029.6051718
AACGCAG13250.029.4303076
CATGGGG8900.029.0338954
GTATAAT1154.183805E-628.6119731
ATCAACG14200.027.4613783
ATGGGAG5750.026.9636525
CAACGCA14500.026.8932135
Produced by FastQC (version 0.11.2)