FastQCFastQC Report
Sun 14 Apr 2019
SRR6576676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448383
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10390.23172154162847386No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6840.15254815637524172No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6670.14875675482790382No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6060.1351523139815738No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5470.12199392037610703No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5360.119540660551359No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5360.119540660551359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.11418809366099965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG26950.034.7940561
TACATGG26650.034.5625422
ACATGGG26650.033.8571853
CATGGGT6400.033.042994
GTATCAA11900.032.8656271
CCGTTCA753.2746352E-431.3296559
CATGGGG8750.026.8539894
CCGATAC700.00883543726.8539893
ACTAGAC700.00883543726.8539893
AACGCAG14350.026.8539896
ATGGGAG6150.026.7448275
ATCAACG15200.025.6614573
CATGGGA15600.025.6059674
ATGGGTG3500.025.511295
CACTCTA3500.025.511299
CAACGCA15300.025.4937385
TCAACGC15300.025.4937384
ATGGGTA4150.024.9127375
GTATTAG2701.8189894E-1224.4329031
TACCGTC1351.4561894E-524.3675087
Produced by FastQC (version 0.11.2)