FastQCFastQC Report
Sun 14 Apr 2019
SRR6576674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319265
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5730.17947473102281802No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4010.12560098977338574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.11777050412666594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3420.10712104364712699No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3280.1027359716849639No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.10179631340735751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.043.2748531
ATATAGC802.4467408E-741.1343883
AACGCAG10650.035.3076976
CATGGGT4850.034.894564
CAACGCA11400.034.2217525
TCAACGC11350.033.96374
ATCAACG11350.033.5495073
TACATGG18700.032.4298632
GGTACCT3100.031.8360048
ACATGGG19050.031.3404883
ATAACGC753.2659082E-431.3404873
GGGTACC3150.031.3306717
GTACATG19500.031.1971571
ATGGGTA3350.030.8678865
ACGCAGA12700.029.2337367
GTATAAC650.006047289429.020611
TAACGCC650.00614074728.9296824
TATCAAC13100.028.7088432
CGCAGAG12850.028.1610328
CGTGACC700.00883029926.8548694
Produced by FastQC (version 0.11.2)