FastQCFastQC Report
Sun 14 Apr 2019
SRR6576673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306585
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8130.26517931405646067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5660.18461438100363683No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5010.16341308283184108No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4590.14971378247468076No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4360.14221178466004536No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4320.14090708938793484No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4290.13992856793385194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.12786013666682977No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3880.12655544139471925No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3530.1151393577637523No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3520.11481318394572466No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3340.1089420552212274No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3270.106658838495034No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3230.1053541432229235No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3100.10111388358856434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.037.4238931
ACATGGG18850.036.1490173
GTACATG19250.035.6769071
TACATGG19850.034.3279082
CATGGGC3450.032.6912844
CATGGGG7350.031.9685174
TTGACGG600.004142408331.35471594
AACGCAG10800.031.3291476
TCAACGC11450.029.5506364
ATCAACG11350.029.3969543
AACGGTC404.7820507E-429.37107584-85
TGGGTAC3050.029.2747766
CATGGGT4850.029.0682814
CAACGCA11450.028.7297865
ATGGGGA4200.027.972455
GTATAAC856.7710475E-427.6704441
ATGGGTA3400.027.6433665
GTATTAC700.00878977326.879861
AACCGAT350.00883862226.85355482-83
ATACCGT700.00883185426.8535546
Produced by FastQC (version 0.11.2)