FastQCFastQC Report
Sun 14 Apr 2019
SRR6576673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306585
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10070.3284570347538203No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6440.21005593880979173No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5800.1891808144560236No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5360.17482916646280802No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4500.14677821811243214No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4180.13634065593554806No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4000.1304695272110508No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3850.12557691994063638No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3750.12231518176036009No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3610.11774874830797331No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3390.11057292431136552No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3340.1089420552212274No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3260.10633266467700637No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3240.10568031704095111No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3150.10274475267870248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG450.001014097741.7668349
TATGCGC250.001703599637.5901564-65
ATAGTAT500.001702271237.590153
GTATCAA10450.036.8827481
GTACATG19200.034.762651
TACATGG19750.033.0806052
CATGGGT5050.032.5657274
ATGGGTA3650.032.1833465
ACATGGG20000.031.9516263
AACGCAG12100.030.677916
TGGGTAC4100.029.797076
GGTACCT3850.029.2910238
ATCAACG12700.029.2285613
GTACTAG650.00614529528.9249341
TCAACGC12850.028.8873714
CAACGCA12900.028.7754045
GGGTACC4000.028.1926127
GACACCG856.815043E-427.6398165
ACGCAGA13500.027.4964987
ATAGACA1205.836757E-627.4094854
Produced by FastQC (version 0.11.2)