FastQCFastQC Report
Sun 14 Apr 2019
SRR6576669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187257
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.27075089315753215No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4610.2461857233641466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3060.16341178166904308No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2490.13297233214245663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.12763207783954672No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2350.12549597611838276No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2250.12015572181547285No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2030.10840716234907107No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1980.10573703519761611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.10520300976732512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT551.2798409E-859.81024
ATTCGCC250.00523198156.392474
GTACTGT608.802726E-539.1614386
GTATCAA9300.034.4531
AGTACTG902.5847923E-531.3291515
CTGTGCG753.2677193E-431.3291519
ACTGTGC1201.6417107E-731.329158
ACATGGG13900.030.7656763
TACATGG14350.030.4639972
GTACATG14600.030.0145051
CTAGTAC1102.9540624E-629.90513
CTCGATA404.7785355E-429.37107764-65
ACACTGC804.769517E-429.3710776
TAGGCAT804.769517E-429.3710775
CGCAAGT404.7785355E-429.37107776-77
ATCAACG11150.028.2383843
CAACGCA11200.028.1123165
TCAACGC11150.027.8169164
CATGGGA8300.027.1770944
GTACTAG700.00870887126.9254551
Produced by FastQC (version 0.11.2)