FastQCFastQC Report
Sun 14 Apr 2019
SRR6576662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377586
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9830.260338042194361No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6580.1742649356702844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.15625579338216988No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5400.14301377699385043No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4890.12950692027776453No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4820.1276530379833998No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4810.12738819765563342No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4680.12394527339467036No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4490.11891330716710893No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4390.11626490388944506No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4230.11202745864518282No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3950.10461192946772391No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3850.10196352619006001No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3780.10010964389569528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.045.4333531
TACATGG23450.043.690992
GTACATG23700.043.2989351
ACATGGG24300.042.162713
ATGGGTA4400.040.589085
GTCGCGT704.6073746E-640.31059394
CATGGGT6500.037.5983054
CATGGGA12600.036.9269034
AACGCAG11850.036.0864226
CAACGCA12050.035.88225
TCAACGC12400.034.8693964
ATCAACG12550.034.452633
GGGTACC4050.033.6483467
ATGGGAG5850.032.9386865
GATATAG1755.456968E-1232.278421
GGTACCT4300.031.7130478
TGGGTAC4750.031.6575366
CATGGGG8150.030.563044
CGCAGAG14200.029.8031798
ACGCAGA14600.028.9674597
Produced by FastQC (version 0.11.2)