FastQCFastQC Report
Sun 14 Apr 2019
SRR6576661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416847
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10210.24493399256801657No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6730.16145012438616568No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6050.14513718462649366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.1367408185737213No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5250.1259454907915854No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4850.11634964387413128No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4770.11443047449064045No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4650.1115517204154042No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4490.10771338164842256No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4390.10531441991905903No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4360.10459473140024998No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4350.10435483522731363No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4170.10003670411445925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8950.054.788291
GTACATG25350.041.2907181
TACATGG26350.039.778172
ACATGGG27200.038.36233
TCAACGC13600.035.2518464
CATGGGA13450.034.9460644
ATCAACG13500.034.816643
CAACGCA14150.033.881635
AACGCAG14450.033.1782076
ATGGGAG6850.032.2498635
CATGGGG8550.031.3349724
GGTATCA3600.030.1233161
CGCAGAG17350.027.6292558
ACGCAGA17050.027.5641177
TAGACGA1205.834545E-627.414819
TATCAAC17550.027.3176692
CATGGGT8450.027.2558654
ATGGGAT5400.026.9828935
ATGGGTA5450.025.8729135
GGTACCT5050.025.1270148
Produced by FastQC (version 0.11.2)