FastQCFastQC Report
Sun 14 Apr 2019
SRR6576659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496204
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9940.20032083578528187No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6890.13885418094170945No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5630.11346139894075824No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5600.11285680889311653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.10802008851198297No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5120.1031833681308494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.040.2054861
TACATGG29100.035.854172
GTACATG29350.035.663771
ACATGGG28950.034.5788653
AACGCAG15550.034.1529126
ATCAACG15200.034.0117383
TCAACGC15800.032.720154
CAACGCA16000.032.604895
CATGGGA15100.032.058264
TATACTG1602.1282176E-929.3737725
ACGCAGA18750.028.5748047
TATCAAC18650.028.2240222
CGCAGAG19100.028.0511848
ATGGGAG7350.027.4954475
CATGGGG10600.026.6026634
ATTAGCC909.5123495E-426.110023
CATGGGT7500.025.6922594
GTCTAGA1301.0596694E-525.38851
GGTACCT4750.024.7358078
GTATAAT1151.3462147E-424.6000371
Produced by FastQC (version 0.11.2)