FastQCFastQC Report
Sun 14 Apr 2019
SRR6576658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329315
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10370.31489607214976545No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5520.16762066714240165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.15456326010051166No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5050.15334861758498702No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5010.15213397506946236No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4810.1460607624918391No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4350.13209237356330567No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4290.13027040979001867No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4290.13027040979001867No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4270.12966308853225636No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3960.1202496090369403No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3880.11782032400589101No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3820.11599836023260404No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3570.10840684451057499No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3560.1081031838816938No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3480.10567389885064452No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3430.1041555957062387No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3360.10202997130407059No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3310.10051166815966475No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3300.1002080075307836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG451.6121401E-552.21825894
GTATCAA8950.046.769391
GGGTACC3800.034.6289527
GGTACCT3900.033.7410288
TGGGTAC4050.033.6517686
GATTTAT702.1728872E-433.5943871
ATGGGTA4250.033.173955
TACATGG18450.031.84042
CAACGCA12450.031.7084375
AACGCAG12750.031.6995546
TCAACGC12500.031.5816024
ATCAACG12550.031.455783
ACCTAAG1052.048495E-631.354761
GTACATG18750.031.3547571
CATGGGT5850.030.5275964
ACATGGG18650.030.490983
CTAAGAC1154.1869243E-628.6065243
TATTATC1154.1869243E-628.6065242
CATGGGG9400.026.997954
GTACCTG5150.026.4640089
Produced by FastQC (version 0.11.2)